.jpg)
|
BEAM
group
(Bioinstrumentation Engineering
And Microanalysis)
|
|
Tissue
Spectroscopy
Investigators:
Lily Hsu Laiho, Peter So |
||||||||||||||||||||||||
|
Tissue
spectroscopy is a powerful method to identify and characterize endogenous
fluorescence species. The relative abundance of these species is
related to physiological and pathological states of the tissue.
Fluorescence spectroscopy has been used to characterize different
tissue types such as colon, lung, cervix, and skin. In addition
to tissue type characterization, spectroscopy has been used to monitor
the physiological state of a tissue. The differences in tissue excitation
spectra has been used to for disease diagnosis such as distinguishing
between malignant and normal tissues.
|
||||||||||||||||||||||||
|
|
Figure 1. Representative spectral data and emission spectra for dermal layer in human skin. | |||||||||||||||||||||||
|
We
have combined both two-photon imaging with spectroscopy in order
to extract information about the chemical components contributing
to the autofluorescence that we see in tissue. The analysis of tissue
spectroscopic information with 3-D microscopic resolution has major
advantages compared with typical tissue spectroscopy methods. Using
different analysis techniques such as multivariate curve resolution,
we are able to extract both the excitation and emission spectrum
of the different components, such as collagen, elastin, NADH, melanin,
and tryptophan. In addition, we are able to obtain the bulk emission
spectrum and examine the spectrum from different layers and individual
structures within the tissue.
|
||||||||||||||||||||||||
 |
Figure 2. Representative spectral data, excitation spectra, and concentration profile for basal cells and elastin in human skin. | |||||||||||||||||||||||
 |
Figure 3. Schematic of bulk, layer, and structure resolved emission spectrum. | |||||||||||||||||||||||
| 1
- Alfano R.R., Tata D., Cordero J., Tomashefsky P., Longo F., Alfano
M. (1984) Laser induced fluorescence spectroscopy from native cancerous
and normal tissues. Inst Electr Electron Eng J Quant Elect.
QE-20: 1507-1511. 2 - Andrew J.J., Hancewicz T.M. (1998) Rapid analysis of raman image data using two-way multivariate curve resolution. Applied Spectroscopy, 52 (6): 797-807. 3 - Schantz S.P., Savage H.E., Sacks P., Alfano R.R. (1997) Native cellular fluorescence and its application to cancer prevention. Environmental Health Perspectives, 105: 941-944. |
||||||||||||||||||||||||
nu} {Main body}------------------------------------[
[So
Lab | Research | People
| Documents | Facilities
| Resource | Links
| Home | Contact
] 